Protein-enhanced fluorescein chemiluminescence used in an immunoassay for rubella antibody in serum.

نویسندگان

  • R Shapiro
  • J Chan
  • A Pierson
  • K Vaccaro
  • J Quick
چکیده

The chemiluminescence of fluorescein derivatives is greatly enhanced when they are conjugated to proteins. We exploited this property in devising a chemiluminescence immunoassay for rubella antibodies. In this sequential "sandwich"-type assay, tubes coated with rubella antigen are used for antibody separation and goat antibody to human IgG conjugated with fluorescein isothiocyanate is used for detection of bound rubella antibodies.

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عنوان ژورنال:
  • Clinical chemistry

دوره 30 6  شماره 

صفحات  -

تاریخ انتشار 1984